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1971 1
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1982 3
1983 1
1984 2
1985 2
1986 5
1987 4
1988 4
1990 2
1991 3
1992 3
1993 2
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1998 3
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2000 3
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74 results

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Page 1
Mutants of β2-glycoprotein I: Their features and potent applications.
Shen L, Azmi NU, Tan XW, Yasuda S, Wahyuningsih AT, Inagaki J, Kobayashi K, Ando E, Sasaki T, Matsuura E. Shen L, et al. Best Pract Res Clin Rheumatol. 2018 Aug;32(4):572-590. doi: 10.1016/j.berh.2019.01.007. Epub 2019 Feb 26. Best Pract Res Clin Rheumatol. 2018. PMID: 31174826 Review.
In culture system, all DV and DI mutants potently inhibited HUVEC's proliferation by 18-30% as compared to control. Only DI and nicked beta2GPI showed significant inhibition in HUVEC's tube formation. Moreover, DV(PE)-coated affinity columns demonstrated its binding …
In culture system, all DV and DI mutants potently inhibited HUVEC's proliferation by 18-30% as compared to control. Only DI and nicked …
Disease-specific expression of tartrate-resistant acid phosphatase isoforms.
Janckila AJ, Nakasato YR, Neustadt DH, Yam LT. Janckila AJ, et al. J Bone Miner Res. 2003 Oct;18(10):1916-9. doi: 10.1359/jbmr.2003.18.10.1916. J Bone Miner Res. 2003. PMID: 14584907 Free article. Review.
We have used simultaneous immunoassays for type-5 TRACP activity and total type-5 TRACP protein in conjunction with non-denaturing gel electrophoresis and column chromatography to investigate the nature and significance of TRACP isoforms 5a and 5b in end-stage renal diseas …
We have used simultaneous immunoassays for type-5 TRACP activity and total type-5 TRACP protein in conjunction with non-denaturing gel elect …
Cathepsin D: the lysosomal aspartic proteinase.
Barrett AJ. Barrett AJ. Ciba Found Symp. 1979;(75):37-50. doi: 10.1002/9780470720585.ch3. Ciba Found Symp. 1979. PMID: 399896 Review.
Cathepsin D was originally known simply as 'cathepsin' and was first purified in the late 1930s. Nowadays the enzyme is purified by conventional column chromatography, and by isoelectric focusing (which resolves isoforms), but affinity chromatography with pepstatin--Sephar …
Cathepsin D was originally known simply as 'cathepsin' and was first purified in the late 1930s. Nowadays the enzyme is purified by conventi …
Expression and Purification of BsaXI Restriction Endonuclease and Engineering New Specificity From BsaXI Specificity Subunit.
Gidwani S, Heiter D, Xu SY. Gidwani S, et al. Front Microbiol. 2022 May 19;13:888435. doi: 10.3389/fmicb.2022.888435. eCollection 2022. Front Microbiol. 2022. PMID: 35663886 Free PMC article.
The BsaXI activity was successfully reconstituted by mixing the BsaXI RM fusion subunit with the BsaXI S subunit and the enzyme complex further purified by chromatography over 6 columns. As expected, the S subunit consisted of two subdomains encoding TRD1-CR1 [target recog …
The BsaXI activity was successfully reconstituted by mixing the BsaXI RM fusion subunit with the BsaXI S subunit and the enzyme complex furt …
Development of a nicking endonuclease-assisted method for the purification of minicircles.
Alves CP, Šimčíková M, Brito L, Monteiro GA, Prazeres DM. Alves CP, et al. J Chromatogr A. 2016 Apr 22;1443:136-44. doi: 10.1016/j.chroma.2016.03.035. Epub 2016 Mar 17. J Chromatogr A. 2016. PMID: 27016116
In this proof-of-concept study, a reproducible process is described to improve the purification of supercoiled (sc) MCs that combines an in vitro enzymatic relaxation of sc MP impurities with topoisomer separation and RNA clearance by hydrophobic interaction chromatography (HIC) …
In this proof-of-concept study, a reproducible process is described to improve the purification of supercoiled (sc) MCs that combines an in …
Purification of the T4 endonuclease V.
Higgins KM, Lloyd RS. Higgins KM, et al. Mutat Res. 1987 Mar;183(2):117-21. doi: 10.1016/0167-8817(87)90053-8. Mutat Res. 1987. PMID: 3547104
The purification of the enzyme was monitored by pyrimidine dimer-specific nicking activity, Western blot analysis and silver or Coomassie Blue staining of SDS-polyacrylamide gels. ...After sonication of cells and removal of major cell debris, total protein and nucleic acid …
The purification of the enzyme was monitored by pyrimidine dimer-specific nicking activity, Western blot analysis and silver or Cooma …
A nicked beta-subunit of human chorionic gonadotropin purified from pregnancy urine.
Sakakibara R, Miyazaki S, Ishiguro M. Sakakibara R, et al. J Biochem. 1990 Jun;107(6):858-62. doi: 10.1093/oxfordjournals.jbchem.a123138. J Biochem. 1990. PMID: 1697291 Free article.
It was found that this beta-subunit consisted of two polypeptide chains composed of residues 1-47 disulfide-bridged to residues 48-145 of the beta-subunit, which may be produced by nicking of the beta-subunit at the one site (Gly47-Val48). This beta-subunit was termed a …
It was found that this beta-subunit consisted of two polypeptide chains composed of residues 1-47 disulfide-bridged to residues 48-145 of th …
Plasmid RK2 ParB protein: purification and nuclease properties.
Johnson EP, Mincer T, Schwab H, Burgin AB, Helinski DR. Johnson EP, et al. J Bacteriol. 1999 Oct;181(19):6010-8. doi: 10.1128/JB.181.19.6010-6018.1999. J Bacteriol. 1999. PMID: 10498713 Free PMC article.
Purification was achieved by treatment of extracts with Polymin P, followed by ammonium sulfate precipitation and heparin and ion-exchange chromatography. Sizing-column analysis indicated that ParB exists as a monomer in solution. Analysis of the enzymatic properties of pu …
Purification was achieved by treatment of extracts with Polymin P, followed by ammonium sulfate precipitation and heparin and ion-exchange c …
Poly(ADP-ribose) synthetase. The DNA binding domain and the automodification domain.
Nishikimi M, Ogasawara K, Kameshita I, Taniguchi T, Shizuta Y. Nishikimi M, et al. J Biol Chem. 1982 Jun 10;257(11):6102-5. J Biol Chem. 1982. PMID: 6281257 Free article.
Their molecular weights are 74,000 and 46,000, respectively, as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The fragment of Mr 46,000 binds to a nicked DNA-cellulose column with the same affinity as that of native enzyme, while the fragment …
Their molecular weights are 74,000 and 46,000, respectively, as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The fra …
A major portion of the 150-kilodalton insulin-like growth factor-binding protein (IGFBP) complex in adult rat serum contains unoccupied, proteolytically nicked IGFBP-3 that binds IGF-II preferentially.
Lee CY, Rechler MM. Lee CY, et al. Endocrinology. 1995 Feb;136(2):668-78. doi: 10.1210/endo.136.2.7530649. Endocrinology. 1995. PMID: 7530649
IGF-I affinity chromatography was used to separate unoccupied 150-kDa complexes that bound to the column and were eluted with acid from complexes that contained endogenous IGF-I, did not bind to the column, and appeared in the flow-through. ...We conclude that the 1 …
IGF-I affinity chromatography was used to separate unoccupied 150-kDa complexes that bound to the column and were eluted with acid fr …
74 results